Facilities

Three laboratories located in the Center for Engineering Education and Research (CEER) building support the majority of the activities in the EMB.

The Core Genomics Laboratory (CEER 106 and 108)

The CG lab is an interdisciplinary facility that provides support for faculty and students, across all engineering fields, engaged in molecular and cellular bioengineering research and teaching. This 700-ft2 laboratory was established in 2009. The state-of-the-art genomics instrumentation in the laboratory was funded by an NSF Major Research Instrumentation grant, awarded in 2007.

The instrument inventory in the Core Laboratory includes an Applied Biosystems 7300 RT-PCR system for quantitative, real-time PCR analysis; a Shimadzu BioSpec-mini UV-Vis spectrophotometer optimized  for rapid quantification of RNA, DNA, and proteins; a NanoDrop spectrophotometer for quantification of RNA and DNA in microliter sample volumes; a microscopy suite with a Leica DM2000 upright microscope and a Leica DMI3000 inverted microscope, equipped with epifluorescent illumination and a Q-Imaging Retiga-SRV cooled CCD camera for imaging of cells and molecular probes; an Eppendorf 5417R refrigerated high-speed mini centrifuge; a Milli-Q Biocell water purification system that supplies RNAase free water; a Beckman-Coulter Vi-CELLTM cell viability analyzer; a Molecular Dynamics Gemini EM fluorescence plate reader; a Shimadzu HPLC with UV-Vis detector; a sonicator for cell lysis; an ultra-low freezer (-85oC); a basic freezer (-20oC); and a crushed ice maker. 

One of the Hewlett Packard 6890 Gas Chromatographs in the Analytical Laboratory (Photo by Nalan Tepe)
One of the Hewlett Packard 6890 Gas Chromatographs in the Analytical Laboratory (Photo by Nalan Tepe)

The Analytical Laboratory (CEER 311)

The Analytical lab is a shared facility providing analytical instrument support for the faculty and students engaged in research in any area of environmental engineering. This state-of the-art facility is also used by a number of faculty members in the College of Engineering as well as faculty from the Chemistry Department. The equipment inventory includes one Hewlett Packard gas chromatograph with mass spectrophotometer detector (GC/MS) with ion and electron ionization capabilities; two Hewlett Packard 6890 gas chromatographs with flame ionization, thermal conductivity, and electron capture detectors; one Dionex DX-600 IC/HPLC with electrochemical, photodiode array, and tandem mass spectrophotometer-mass spectrophotometer (MS/MS) detectors; and a Spectronic Genesys 5 variable wavelength scanning UV/Vis spectrophotometer.  

The Environmental Microbiology and Biotechnology Laboratory (CEER 306)

The EMB lab is equipped with standard laboratory equipment such as ovens and pH meters, the laboratory is used for teaching undergraduate level environmental engineering laboratory courses and for research. The laboratory has a 110-ft2 environmental room maintained at 35 ±2oC for research related to anaerobic processes. Recently, a strong analytical infrastructure has been established in CEER 306 to incorporate several molecular biology tools, including PCR, FAME, and gel electrophoresis, into its research projects, particularly in the microbial source tracking and the microbial community structure-function analyses areas.